TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Rajendran, Lawrence
A1  - Beckmann, Julia
A1  - Magenau, Astrid
A1  - Boneberg, Eva-Maria
A1  - Gaus, Katharina
A1  - Viola, Antonella
A1  - Giebel, Bernd
A1  - Illges, Harald
T1  - Flotillins Are Involved in the Polarization of Primitive and Mature Hematopoietic Cells
JF  - PLoS ONE
N2  - Background: Migration of mature and immature leukocytes in response to chemokines is not only essential during inflammation and host defense, but also during development of the hematopoietic system. Many molecules implicated in migratory polarity show uniform cellular distribution under non-activated conditions, but acquire a polarized localization upon exposure to migratory cues.
Methodology/Principal Findings: Here, we present evidence that raft-associated endocytic proteins (flotillins) are preassembled in lymphoid, myeloid and primitive hematopoietic cells and accumulate in the uropod during migration. Furthermore, flotillins display a polarized distribution during immunological synapse formation. Employing the membrane lipid-order sensitive probe Laurdan, we show that flotillin accumulation in the immunological synapse is concomittant with membrane ordering in these regions.
Conclusions: Together with the observation that flotillin polarization does not occur in other polarized cell types such as polarized epithelial cells, our results suggest a specific role for flotillins in hematopoietic cell polarization. Based on our results, we propose that in hematopoietic cells, flotillins provide intrinsic cues that govern segregation of certain microdomain-associated molecules during immune cell polarization.
UN  - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-36792
SN  - 1932-6203
SS  - 1932-6203
U6  - https://doi.org/10.1371/journal.pone.0008290
DO  - https://doi.org/10.1371/journal.pone.0008290
PM  - 20027317
VL  - 4
IS  - 12
SP  - 1
EP  - 12
PB  - Public Library of Science (PLoS)
CY  - San Francisco, CA, USA
ER  -