TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Klett, M. V.
A1  - Boneberg, E-M
A1  - Trenz, K.
A1  - Knippers, R.
A1  - Illges, H.
T1  - Hydrostatic pressure induces apoptosis in the human leukaemic T-cell line Jurkat via the mitochondrial pathway
JF  - Scand J Immunol. (Scandinavian Journal of Immunology)
N2  - We investigated the effect of pressure levels ranging from 80 to 500 bar on the proliferative capacity and viability of Jurkat leukaemic T cells. Pressurization at 360 bar induced apoptotic cell death as shown by apoptotic morphology after Hoechst staining, DNA fragmentation in the TdT-mediated dUTP nick end labelling-assay and cleavage of several caspase substrates. Cell death could be prevented by the general caspase inhibitor zVAD-fmk. Breakdown of the mitochondrial membrane potential and the release of cytochrome c provided strong evidence for an involvement of the mitochondrial pathway, whereas a central role of the death receptor pathway was excluded because caspase-8 was not significantly activated. Pressure incubation led to calcium influx after 5 min, and we hypothesize that calcium influx could be the primary trigger for pressure-induced apoptosis.
SN  - 0300-9475
SS  - 0300-9475
U6  - https://doi.org/10.1111/j.0300-9475.2004.01496.x
DO  - https://doi.org/10.1111/j.0300-9475.2004.01496.x
PM  - 15379865
VL  - 60
IS  - 4
SP  - 403
EP  - 411
PB  - Blackwell Publishing
ER  -