TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Möller, Steffen A1 - Mix, Eilhard A1 - Blüggel, Martin A1 - Serrano-Fernández, Pablo A1 - Koczan, Dirk A1 - Kotsikoris, Vasilis A1 - Kunz, Manfred A1 - Watson, Michael A1 - Pahnke, Jens A1 - Illges, Harald A1 - Kreutzer, Michael A1 - Mikkat, Stefan A1 - Thiesen, Hans-Jürgen A1 - Glocker, Michael O. A1 - Zettl, Uwe K. A1 - Ibrahim, Saleh M. T1 - Collection of soluble variants of membrane proteins for transcriptomics and proteomics JF - In Silico Biol. (In Silico Biology) N2 - The existence of a soluble splice variant for a gene encoding a transmembrane protein suggests that this gene plays a role in intercellular signalling, particularly in immunological processes. Also, the absence of a splice variant of a reported soluble variant suggests exclusive control of the solubilisation by proteolytic cleavage. Soluble splice variants of membrane proteins may also be interesting targets for crystallisation as their structure may be expected to preserve, at least partially, their function as integral membrane proteins, whose structures are most difficult to determine. This paper presents a dataset derived from the literature in an attempt to collect all reported soluble variants of membrane proteins, be they splice variants or shedded. A list of soluble variants is derived in silico from Ensembl. These are checked on their presence in multiple organisms and their number of membranespanning regions is inspected. The findings then are confirmed by a comparison with identified proteins of a recent global proteomics study of human blood plasma. Finally, a tool to determine novel soluble variants by proteomics is provided. UR - https://content.iospress.com/articles/in-silico-biology/isb00188 SN - 1386-6338 SS - 1386-6338 PM - 15984938 VL - 5 IS - 3 SP - 295 EP - 311 PB - IOS Press ER -