TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Möller, Steffen
A1  - Mix, Eilhard
A1  - Blüggel, Martin
A1  - Serrano-Fernández, Pablo
A1  - Koczan, Dirk
A1  - Kotsikoris, Vasilis
A1  - Kunz, Manfred
A1  - Watson, Michael
A1  - Pahnke, Jens
A1  - Illges, Harald
A1  - Kreutzer, Michael
A1  - Mikkat, Stefan
A1  - Thiesen, Hans-Jürgen
A1  - Glocker, Michael O.
A1  - Zettl, Uwe K.
A1  - Ibrahim, Saleh M.
T1  - Collection of soluble variants of membrane proteins for transcriptomics and proteomics
JF  - In Silico Biol. (In Silico Biology)
N2  - The existence of a soluble splice variant for a gene encoding a transmembrane protein suggests that this gene plays a role in intercellular signalling, particularly in immunological processes. Also, the absence of a splice variant of a reported soluble variant suggests exclusive control of the solubilisation by proteolytic cleavage. Soluble splice variants of membrane proteins may also be interesting targets for crystallisation as their structure may be expected to preserve, at least partially, their function as integral membrane proteins, whose structures are most difficult to determine. This paper presents a dataset derived from the literature in an attempt to collect all reported soluble variants of membrane proteins, be they splice variants or shedded. A list of soluble variants is derived in silico from Ensembl. These are checked on their presence in multiple organisms and their number of membranespanning regions is inspected. The findings then are confirmed by a comparison with identified proteins of a recent global proteomics study of human blood plasma. Finally, a tool to determine novel soluble variants by proteomics is provided.
UR  - https://content.iospress.com/articles/in-silico-biology/isb00188
SN  - 1386-6338
SS  - 1386-6338
PM  - 15984938
VL  - 5
IS  - 3
SP  - 295
EP  - 311
PB  - IOS Press
ER  -