TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Neidhöfer, Claudio
A1  - Sib, Esther
A1  - Benhsain, Al-Harith
A1  - Mutschnik-Raab, Christina
A1  - Schwabe, Anna
A1  - Wollkopf, Alexander
A1  - Wetzig, Nina
A1  - Sieber, Martin A.
A1  - Thiele, Ralf
A1  - Döhla, Manuel
A1  - Engelhart, Steffen
A1  - Mutters, Nico T.
A1  - Parčina, Marijo
T1  - Examining Different Analysis Protocols Targeting Hospital Sanitary Facility Microbiomes
JF  - Microorganisms
N2  - Indoor spaces exhibit microbial compositions that are distinctly dissimilar from one another and from outdoor spaces. Unique in this regard, and a topic that has only recently come into focus, is the microbiome of hospitals. While the benefits of knowing exactly which microorganisms propagate how and where in hospitals are undoubtedly beneficial for preventing hospital-acquired infections, there are, to date, no standardized procedures on how to best study the hospital microbiome. Our study aimed to investigate the microbiome of hospital sanitary facilities, outlining the extent to which hospital microbiome analyses differ according to sample-preparation protocol. For this purpose, fifty samples were collected from two separate hospitals—from three wards and one hospital laboratory—using two different storage media from which DNA was extracted using two different extraction kits and sequenced with two different primer pairs (V1–V2 and V3–V4). There were no observable differences between the sample-preservation media, small differences in detected taxa between the DNA extraction kits (mainly concerning Propionibacteriaceae), and large differences in detected taxa between the two primer pairs V1–V2 and V3–V4. This analysis also showed that microbial occurrences and compositions can vary greatly from toilets to sinks to showers and across wards and hospitals. In surgical wards, patient toilets appeared to be characterized by lower species richness and diversity than staff toilets. Which sampling sites are the best for which assessments should be analyzed in more depth. The fact that the sample processing methods we investigated (apart from the choice of primers) seem to have changed the results only slightly suggests that comparing hospital microbiome studies is a realistic option. The observed differences in species richness and diversity between patient and staff toilets should be further investigated, as these, if confirmed, could be a result of excreted antimicrobials.
KW  - hospital environment
KW  - hospital-acquired infections
KW  - built environment
KW  - 16S rRNA gene sequencing
KW  - NGS
KW  - high-throughput DNA sequencing
KW  - microbial ecology
KW  - microbiome
UN  - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-65910
SN  - 2076-2607
SS  - 2076-2607
U6  - https://doi.org/10.3390/microorganisms11010185
DO  - https://doi.org/10.3390/microorganisms11010185
PM  - 36677477
VL  - 11
IS  - 1
SP  - 11
S1  - 11
PB  - MDPI
CY  - Basel
ER  -