TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Rumpf, Jessica
A1  - Burger, René
A1  - Schulze, Margit
T1  - Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins
JF  - International Journal of Biological Macromolecules
N2  - This research studies in detail four different assays, namely DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), FRAP (ferric ion reducing antioxidant potential) and FC (Folin-Ciocalteu), to determine the antioxidant capacity of standard substances as well as 50 organosolv lignins, and two kraft lignins. The coefficient of variation was determined for each method and was lowest for ABTS and highest for DPPH. The best correlation was found for FRAP and FC, which both rely on a single electron transfer mechanism. A good correlation between ABTS, FRAP and FC, respectively, could be observed, even though ABTS relies on a more complex reaction mechanism. The DPPH assay merely correlates with the others, implying that it reflects different antioxidative attributes due to a different reaction mechanism. Lignins obtained from paulownia and silphium have been investigated for the first time regarding their antioxidant capacity. Paulownia lignin is in the same range as beech wood lignin, while silphium lignin resembles wheat straw lignin. Miscanthus lignin is an exception from the grass lignins and possesses a significantly higher antioxidant capacity. All lignins possess a good antioxidant capacity and thus are promising candidates for various applications, e. g. as additives in food packaging or for biomedical purposes.
KW  - Lignin
KW  - Antioxidant capacity
KW  - Assay reproducibility
Y1  - 2023
UN  - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-66029
SN  - 0141-8130
SS  - 0141-8130
U6  - https://doi.org/10.1016/j.ijbiomac.2023.123470
DO  - https://doi.org/10.1016/j.ijbiomac.2023.123470
PM  - 36736974
VL  - 233
SP  - 9
S1  - 9
PB  - Elsevier
ER  -