TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Lawong, Rene Y.
A1  - May, Fabian
A1  - Etang, Etang C.
A1  - Vorrat, Philipp
A1  - George, Jonas
A1  - Weder, Julia
A1  - Kockler, Dagmar
A1  - Preller, Matthias
A1  - Althaus, Mike
T1  - Recording Sodium Self-Inhibition of Epithelial Sodium Channels Using Automated Electrophysiology in Xenopus Oocytes
JF  - Membranes
N2  - The epithelial sodium channel (ENaC) is a key regulator of sodium homeostasis that contributes to blood pressure control. ENaC open probability is adjusted by extracellular sodium ions, a mechanism referred to as sodium self-inhibition (SSI). With a growing number of identified ENaC gene variants associated with hypertension, there is an increasing demand for medium- to high-throughput assays allowing the detection of alterations in ENaC activity and SSI. We evaluated a commercially available automated two-electrode voltage-clamp (TEVC) system that records transmembrane currents of ENaC-expressing Xenopus oocytes in 96-well microtiter plates. We employed guinea pig, human and Xenopus laevis ENaC orthologs that display specific magnitudes of SSI. While demonstrating some limitations over traditional TEVC systems with customized perfusion chambers, the automated TEVC system was able to detect the established SSI characteristics of the employed ENaC orthologs. We were able to confirm a reduced SSI in a gene variant, leading to C479R substitution in the human α-ENaC subunit that has been reported in Liddle syndrome. In conclusion, automated TEVC in Xenopus oocytes can detect SSI of ENaC orthologs and variants associated with hypertension. For precise mechanistic and kinetic analyses of SSI, optimization for faster solution exchange rates is recommended.
KW  - two-electrode voltage clamp
KW  - ENaC
KW  - automated electrophysiology
KW  - hypertension
KW  - sodium self-inhibition
UN  - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-70317
SN  - 2077-0375
SS  - 2077-0375
U6  - https://doi.org/10.3390/membranes13050529
DO  - https://doi.org/10.3390/membranes13050529
PM  - 37233590
VL  - 13
IS  - 5
SP  - 11
S1  - 11
PB  - MDPI
CY  - Basel
ER  -