TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Jäger, Richard
T1  - Genomic Multicopy Loci Targeted by Current Forensic Quantitative PCR Assays
JF  - Genes
N2  - Modern forensic DNA quantitation assays provide information on the suitability of a DNA extract for a particular type of analysis, on the amount of sample to put into the analysis in order to yield an optimal (or best possible) result, and on the requirement for optional steps to improve the analysis. To achieve a high sensitivity and specificity, these assays are based on quantitative PCR (qPCR) and analyze target DNA loci that are present in multiple copies distributed across the genome. These target loci allow the determination of the amount of DNA, the degree of DNA degradation, and the proportion of DNA from male contributors. In addition, internal control DNA of a known amount is analyzed in order to inform about the presence of PCR inhibitors. These assays are nowadays provided as commercial kits that have been technically validated and are compatible with common qPCR instruments. In this review, the principles of forensic qPCR assays will be explained, followed by information on the nature of DNA loci targeted by modern forensic qPCR assays. Finally, we critically draw attention to the current trend of manufacturers not to disclose the exact nature of the target loci of their commercial kits.
KW  - STR
KW  - TaqMan
KW  - quantitative PCR
KW  - Plexor
KW  - Y chromosome
KW  - qPCR
KW  - Alu
KW  - real-time PCR
KW  - DNA degradation
KW  - forensic
Y1  - 2024
UN  - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-86210
SN  - 2073-4425
SS  - 2073-4425
U6  - https://doi.org/10.3390/genes15101299
DO  - https://doi.org/10.3390/genes15101299
PM  - 39457423
VL  - 15
IS  - 10
SP  - 15
S1  - 15
PB  - MDPI
CY  - Basel
ER  -