@article{KuschDeiningerSpechtetal.2011,
  author    = {Peter Kusch and Susanne Deininger and Sabine Specht and Rudeka Maniako and Stefanie Haubrich and Tanja Pommerening and Paul Kong Thoo Lin and Achim Hoerauf and Annette Kaiser},
  title     = {In Vitro and In Vivo Antimalarial Activity Assays of Seeds from Balanites aegyptiaca: Compounds of the Extract Show Growth Inhibition and Activity against Plasmodial Aminopeptidase},
  series   = {Journal of Parasitology Research},
  volume    = {2011},
  publisher = {Hindawi},
  address   = {London},
  issn      = {2090-0031},
  doi       = {10.1155/2011/368692},
  url       = {https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-8527},
  year      = {2011},
  abstract  = {Balanites aegyptiaca (Balanitaceae) is a widely grown desert plant with multiuse potential. In the present paper, a crude extract from B. aegyptiaca seeds equivalent to a ratio of 1 : 2000 seeds to the extract was screened for antiplasmodial activity. The determined IC(50) value for the chloroquine-susceptible Plasmodium falciparum NF54 strain was 68.26 μg/μL ± 3.5. Analysis of the extract by gas chromatography-mass spectrometry detected 6-phenyl-2(H)-1,2,4-triazin-5-one oxime, an inhibitor of the parasitic M18 Aspartyl Aminopeptidase as one of the compounds which is responsible for the in vitro antiplasmodial activity. The crude plant extract had a K(i) of 2.35 μg/μL and showed a dose-dependent response. After depletion of the compound, a significantly lower inhibition was determined with a K(i) of 4.8 μg/μL. Moreover, two phenolic compounds, that is, 2,6-di-tert-butyl-phenol and 2,4-di-tert-butyl-phenol, with determined IC(50) values of 50.29 μM ± 3 and 47.82 μM ± 2.5, respectively, were detected. These compounds may contribute to the in vitro antimalarial activity due to their antioxidative properties. In an in vivo experiment, treatment of BALB/c mice with the aqueous Balanite extract did not lead to eradication of the parasites, although a reduced parasitemia at day 12 p.i. was observed.},
  language  = {en}
}