TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Witzler, Markus
A1  - Ottensmeyer, Patrick Frank
A1  - Gericke, Martin
A1  - Heinze, Thomas
A1  - Tobiasch, Edda
A1  - Schulze, Margit
T1  - Non-Cytotoxic Agarose/Hydroxyapatite Composite Scaffolds for Drug Release
JF  - International Journal of Molecular Sciences
N2  - Healing of large bone defects requires implants or scaffolds that provide structural guidance for cell growth, differentiation, and vascularization. In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system. Hydroxyapatite (HA) was incorporated into the agarose gels in situ in various ratios by a simple procedure consisting of precipitation, cooling, washing, and drying. The resulting gels were characterized regarding composition, porosity, mechanical properties, and biocompatibility. A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers. Mechanical testing revealed elastic moduli of up to 2.8 MPa for lyophilized composites. MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds. Furthermore, scaffolds were loaded with model drug compounds for guided hMSC differentiation. Different release kinetic models were evaluated for adenosine 5′-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.
KW  - hydroxyapatite
KW  - bone tissue engineering
KW  - hydrogel
KW  - agarose
KW  - biocomposite
KW  - drug release
UN  - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-45564
SN  - 1422-0067
SS  - 1422-0067
U6  - https://doi.org/10.3390/ijms20143565
DO  - https://doi.org/10.3390/ijms20143565
PM  - 31330875
VL  - 20
IS  - 14
PB  - MDPI
CY  - Basel
ER  -