@article{SuterStebelRiannaetal.2020,
  author    = {Naiana Suter and Sophie Stebel and Carmela Rianna and Manfred Radmacher and Dorothea Br{\"u}ggemann},
  title     = {Spatial patterning of nanofibrous collagen scaffolds modulates fibroblast morphology},
  series   = {Biofabrication},
  volume    = {13},
  number    = {1},
  publisher = {IOP Publishing},
  issn      = {1758-5082},
  doi       = {10.1088/1758-5090/abb744},
  pages     = {015007},
  year      = {2020},
  abstract  = {Current knowledge about cell-biomaterial interactions is often based on 2D cell culture systems like protein-coated glass slides. However, such smooth surfaces cannot mimic the nanofibrous environment of the native extracellular matrix (ECM). It is therefore a major challenge to transfer the results from 2D surfaces to 3D protein scaffolds with biomimetic nanofiber architecture. To understand the influence of different protein topographies on the cell response we introduce a new process to fabricate binary collagen scaffolds of variable thickness with spatially controlled regions of nanofibrous and smooth topography. We used pH-induced self-assembly to prepare collagen nanofibers with diameters between 130 and 150 nm on glass surfaces, which were partly covered with a polymer mask. After cross-linking with glutaraldehyde, smooth collagen films were prepared on the remaining glass regions. Atomic force microscopy revealed a much lower surface roughness of smooth collagen compared to nanofibers.},
  language  = {en}
}