TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Grimmig, Roman
A1  - Babczyk, Patrick
A1  - Gillemot, Philipp
A1  - Schmitz, Klaus-Peter
A1  - Schulze, Margit
A1  - Tobiasch, Edda
T1  - Development and Evaluation of a Prototype Scratch Apparatus for Wound Assays Adjustable to Different Forces and Substrates
JF  - Applied Sciences
N2  - Scratch assays enable the study of the migration process of an injured adherent cell layer in vitro. An apparatus for the reproducible performance of scratch assays and cell harvesting has been developed that meets the requirements for reproducibility in tests as well as easy handling. The entirely autoclavable setup is divided into a sample translation and a scratching system. The translational system is compatible with standard culture dishes and can be modified to adapt to different cell culture systems, while the scratching system can be adjusted according to angle, normal force, shape, and material to adapt to specific questions and demanding substrates. As a result, a fully functional prototype can be presented. This system enables the creation of reproducible and clear scratch edges with a low scratch border roughness within a monolayer of cells. Moreover, the apparatus allows the collection of the migrated cells after scratching for further molecular biological investigations without the need for a second processing step. For comparison, the mechanical properties of manually performed scratch assays are evaluated.
KW  - cell harvesting
KW  - endothelial cells
KW  - scratch assay
KW  - wound healing assay
KW  - cell migration
KW  - prototype apparatus
UN  - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-46464
SN  - 2076-3417
SS  - 2076-3417
U6  - https://doi.org/10.3390/app9204414
DO  - https://doi.org/10.3390/app9204414
VL  - 9
IS  - 20
SP  - 11
S1  - 11
PB  - MDPI
CY  - Basel
ER  -