TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Kusch, Peter
A1  - Deininger, Susanne
A1  - Specht, Sabine
A1  - Maniako, Rudeka
A1  - Haubrich, Stefanie
A1  - Pommerening, Tanja
A1  - Kong Thoo Lin, Paul
A1  - Hoerauf, Achim
A1  - Kaiser, Annette
T1  - In Vitro and In Vivo Antimalarial Activity Assays of Seeds from Balanites aegyptiaca: Compounds of the Extract Show Growth Inhibition and Activity against Plasmodial Aminopeptidase
JF  - Journal of Parasitology Research
N2  - Balanites aegyptiaca (Balanitaceae) is a widely grown desert plant with multiuse potential. In the present paper, a crude extract from B. aegyptiaca seeds equivalent to a ratio of 1 : 2000 seeds to the extract was screened for antiplasmodial activity. The determined IC(50) value for the chloroquine-susceptible Plasmodium falciparum NF54 strain was 68.26 μg/μL ± 3.5. Analysis of the extract by gas chromatography-mass spectrometry detected 6-phenyl-2(H)-1,2,4-triazin-5-one oxime, an inhibitor of the parasitic M18 Aspartyl Aminopeptidase as one of the compounds which is responsible for the in vitro antiplasmodial activity. The crude plant extract had a K(i) of 2.35 μg/μL and showed a dose-dependent response. After depletion of the compound, a significantly lower inhibition was determined with a K(i) of 4.8 μg/μL. Moreover, two phenolic compounds, that is, 2,6-di-tert-butyl-phenol and 2,4-di-tert-butyl-phenol, with determined IC(50) values of 50.29 μM ± 3 and 47.82 μM ± 2.5, respectively, were detected. These compounds may contribute to the in vitro antimalarial activity due to their antioxidative properties. In an in vivo experiment, treatment of BALB/c mice with the aqueous Balanite extract did not lead to eradication of the parasites, although a reduced parasitemia at day 12 p.i. was observed.
UN  - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-8527
SN  - 2090-0031
SS  - 2090-0031
SN  - 2090-0023
SS  - 2090-0023
U6  - https://doi.org/10.1155/2011/368692
DO  - https://doi.org/10.1155/2011/368692
PM  - 21687598
VL  - 2011
SP  - 9
S1  - 9
PB  - Hindawi
CY  - London
ER  -