TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Singh, Anjana A1 - Rajasekaran, Narendiran A1 - Hartenstein, Bettina A1 - Szabowski, Sibylle A1 - Gajda, Mieczyslaw A1 - Angel, Peter A1 - Bräuer, Rolf A1 - Illges, Harald T1 - Collagenase-3 (MMP-13) deficiency protects C57BL/6 mice from antibody-induced arthritis JF - Arthritis Research & Therapy N2 - Introduction: Matrix metalloproteinases (MMPs) are important in tissue remodelling. Here we investigate the role of collagenase-3 (MMP-13) in antibody-induced arthritis. Methods: For this study we employed the K/BxN serum-induced arthritis model. Arthritis was induced in C57BL/6 wild type (WT) and MMP-13-deficient (MMP-13–/–) mice by intraperitoneal injection of 200 μl of K/BxN serum. Arthritis was assessed by measuring the ankle swelling. During the course of the experiments, mice were sacrificed every second day for histological examination of the ankle joints. Ankle sections were evaluated histologically for infiltration of inflammatory cells, pannus tissue formation and bone/cartilage destruction. Semi-quantitative PCR was used to determine MMP-13 expression levels in ankle joints of untreated and K/BxN serum-injected mice. Results: This study shows that MMP-13 is a regulator of inflammation. We observed increased expression of MMP-13 in ankle joints of WT mice during K/BxN serum-induced arthritis and both K/BxN serum-treated WT and MMP-13–/– mice developed progressive arthritis with a similar onset. However, MMP-13–/– mice showed significantly reduced disease over the whole arthritic period. Ankle joints of WT mice showed severe joint destruction with extensive inflammation and erosion of cartilage and bone. In contrast, MMP-13–/– mice displayed significantly decreased severity of arthritis (50% to 60%) as analyzed by clinical and histological scoring methods. Conclusions: MMP-13 deficiency acts to suppress the local inflammatory responses. Therefore, MMP-13 has a role in the pathogenesis of arthritis, suggesting MMP-13 is a potential therapeutic target. KW - Articular Cartilage KW - Wild Type Mouse KW - Ankle Joint KW - Joint Destruction KW - Cartilage Destruction UN - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-10819 SN - 1478-6354 SS - 1478-6354 U6 - https://doi.org/10.1186/ar4423 DO - https://doi.org/10.1186/ar4423 PM - 24369907 VL - 15 IS - 6 SP - 7 S1 - 7 PB - BioMed Central ER -