TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Breuch, René
A1  - Klein, Daniel
A1  - Siefke, Eleni
A1  - Hebel, Martin
A1  - Herbert, Ulrike
A1  - Wickleder, Claudia
A1  - Kaul, Peter
T1  - Differentiation of meat-related microorganisms using paper-based surface-enhanced Raman spectroscopy combined with multivariate statistical analysis
JF  - Talanta
N2  - Surface-enhanced Raman spectroscopy (SERS) with subsequent chemometric evaluation was performed for the rapid and non-destructive differentiation of seven important meat-associated microorganisms, namely Brochothrix thermosphacta DSM 20171, Pseudomonas fluorescens DSM 4358, Salmonella enterica subsp. enterica sv. Enteritidis DSM 14221, Listeria monocytogenes DSM 19094, Micrococcus luteus DSM 20030, Escherichia coli HB101 and Bacillus thuringiensis sv. israelensis DSM 5724. A simple method for collecting spectra from commercial paper-based SERS substrates without any laborious pre-treatments was used. In order to prepare the spectroscopic data for classification at genera level with a subsequent chemometric evaluation consisting of principal component analysis and discriminant analysis, a pre-processing method with spike correction and sum normalisation was performed. Because of the spike correction rather than exclusion, and therefore the use of a balanced data set, the multivariate analysis of the data is significantly resilient and meaningful. The analysis showed that the differentiation of meat-associated microorganisms and thereby the detection of important meat-related pathogenic bacteria was successful on genera level and a cross-validation as well as a classification of ungrouped data showed promising results, with 99.5 % and 97.5 %, respectively.
KW  - Raman Spectroscopy
KW  - Pathogenic Bacteria
KW  - Classification
KW  - Meat-associated Microorganisms
KW  - Chemometrics
KW  - SERS
UN  - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-49666
SN  - 0039-9140
SS  - 0039-9140
U6  - https://doi.org/10.1016/j.talanta.2020.121315
DO  - https://doi.org/10.1016/j.talanta.2020.121315
PM  - 32887055
VL  - 219
SP  - 7
S1  - 7
PB  - Elsevier
ER  -