TY - JOUR U1 - Wissenschaftlicher Artikel A1 - Billinger, Kira A1 - Okai, Charles A. A1 - Russ, Manuela A1 - Koy, Cornelia A1 - Röwer, Claudia A1 - Opuni, Kwabena F. M. A1 - Illges, Harald A1 - Pecks, Ulrich A1 - Glocker, Michael O. T1 - Dried serum spots on pre-punched filter paper discs are ready-to-use storage and shipping devices for blood-borne antigens and antibodies JF - Journal of Immunological Methods N2 - Dried serum spots that are well prepared can be attractive alternatives to frozen serum samples for shelving specimens in a medical or research center's biobank and mailing freshly prepared serum to specialized laboratories. During the pre-analytical phase, complications can arise which are often challenging to identify or are entirely overlooked. These complications can lead to reproducibility issues, which can be avoided in serum protein analysis by implementing optimized storage and transfer procedures. With a method that ensures accurate loading of filter paper discs with donor or patient serum, a gap in dried serum spot preparation and subsequent serum analysis shall be filled. Pre-punched filter paper discs with a 3 mm diameter are loaded within seconds in a highly reproducible fashion (approximately 10% standard deviation) when fully submerged in 10 μl of serum, named the "Submerge and Dry" protocol. Such prepared dried serum spots can store several hundred micrograms of proteins and other serum components. Serum-borne antigens and antibodies are reproducibly released in 20 μl elution buffer in high yields (approximately 90%). Dried serum spot-stored and eluted antigens kept their epitopes and antibodies their antigen binding abilities as was assessed by SDS-PAGE, 2D gel electrophoresis-based proteomics, and Western blot analysis, suggesting pre-punched filter paper discs as handy solution for serological tests. KW - Antigen analysis KW - Antibody analysis KW - Submerge and dry protocol KW - Patient serum KW - Dried serum spots KW - Pre-punched filter paper discs KW - Proteome analysis Y1 - 2023 UN - https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-73977 SN - 0022-1759 SS - 0022-1759 U6 - https://doi.org/10.1016/j.jim.2023.113519 DO - https://doi.org/10.1016/j.jim.2023.113519 PM - 37419022 VL - 519 SP - 8 S1 - 8 PB - Elsevier ER -