Flotillins are involved in the polarization of primitive and mature hematopoietic cells

  • BACKGROUND Migration of mature and immature leukocytes in response to chemokines is not only essential during inflammation and host defense, but also during development of the hematopoietic system. Many molecules implicated in migratory polarity show uniform cellular distribution under non-activated conditions, but acquire a polarized localization upon exposure to migratory cues. METHODOLOGY/PRINCIPAL FINDINGS Here, we present evidence that raft-associated endocytic proteins (flotillins) are pre-assembled in lymphoid, myeloid and primitive hematopoietic cells and accumulate in the uropod during migration. Furthermore, flotillins display a polarized distribution during immunological synapse formation. Employing the membrane lipid-order sensitive probe Laurdan, we show that flotillin accumulation in the immunological synapse is concomittant with membrane ordering in these regions. CONCLUSIONS Together with the observation that flotillin polarization does not occur in other polarized cell types such as polarized epithelial cells, our results suggest a specific role for flotillins in hematopoietic cell polarization. Based on our results, we propose that in hematopoietic cells, flotillins provide intrinsic cues that govern segregation of certain microdomain-associated molecules during immune cell polarization.

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Metadaten
Document Type:Article
Language:English
Parent Title (English):PLoS ONE (PloS one)
Volume:4
Issue:12
First Page:e8290
ISSN:1932-6203
DOI:https://doi.org/10.1371/journal.pone.0008290
Publisher:PloS
Date of first publication:2009/12/22
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Institut für funktionale Gen-Analytik (IfGA)
Dewey Decimal Classification (DDC):500 Naturwissenschaften und Mathematik / 570 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Entry in this database:2018/06/06