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Lignocellulosic Biomass as Source for Lignin-Based Environmentally Benign Antioxidants

  • Antioxidant activity is an essential aspect of oxygen-sensitive merchandise and goods, such as food and corresponding packaging, cosmetics, and biomedicine. Technical lignin has not yet been applied as a natural antioxidant, mainly due to the complex heterogeneous structure and polydispersity of lignin. This report presents antioxidant capacity studies completed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The influence of purification on lignin structure and activity was investigated. The purification procedure showed that double-fold selective extraction is the most efficient (confirmed by ultraviolet-visible (UV/Vis), Fourier transform infrared (FTIR), heteronuclear single quantum coherence (HSQC) and 31P nuclear magnetic resonance spectroscopy, size exclusion chromatography, and X-ray diffraction), resulting in fractions of very narrow polydispersity (3.2⁻1.6), up to four distinct absorption bands in UV/Vis spectroscopy. Due to differential scanning calorimetry measurements, the glass transition temperature increased from 123 to 185 °C for the purest fraction. Antioxidant capacity is discussed regarding the biomass source, pulping process, and degree of purification. Lignin obtained from industrial black liquor are compared with beech wood samples: antioxidant activity (DPPH inhibition) of kraft lignin fractions were 62⁻68%, whereas beech and spruce/pine-mixed lignin showed values of 42% and 64%, respectively. Total phenol content (TPC) of the isolated kraft lignin fractions varied between 26 and 35%, whereas beech and spruce/pine lignin were 33% and 34%, respectively. Storage decreased the TPC values but increased the DPPH inhibition.

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Document Type:Article
Author:Abla Alzagameem, Basma El Khaldi-Hansen, Dominik Büchner, Michael Larkins, Birgit Kamm, Steffen Witzleben, Margit Schulze
Parent Title (English):Molecules
Article Number:2664
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=30332854
Place of publication:Basel
Publishing Institution:Hochschule Bonn-Rhein-Sieg
Date of first publication:2018/10/16
Copyright:© 2018 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Funding Information:Financial support via BMBF program “IngenieurNachwuchs” projects "LignoBau" (03FH013IX4).
Keyword:Folin-Ciocalteu assay; antioxidant activity; biomass; kraft lignin; lignocellulose feedstock; organosolv; total phenol content
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Institut für Technik, Ressourcenschonung und Energieeffizienz (TREE)
Projects:LignoBau - IngenieurNachwuchs 2014: Lignin-basierte Polymere für bauchemische Anwendungen (DE/BMBF/03FH013IX4,13FH013IX4)
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Entry in this database:2018/10/24
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International