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Non-Cytotoxic Agarose/Hydroxyapatite Composite Scaffolds for Drug Release

  • Healing of large bone defects requires implants or scaffolds that provide structural guidance for cell growth, differentiation, and vascularization. In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system. Hydroxyapatite (HA) was incorporated into the agarose gels in situ in various ratios by a simple procedure consisting of precipitation, cooling, washing, and drying. The resulting gels were characterized regarding composition, porosity, mechanical properties, and biocompatibility. A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers. Mechanical testing revealed elastic moduli of up to 2.8 MPa for lyophilized composites. MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds. Furthermore, scaffolds were loaded with model drug compounds for guided hMSC differentiation. Different release kinetic models were evaluated for adenosine 5′-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.

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Document Type:Article
Author:Markus Witzler, Patrick Frank Ottensmeyer, Martin Gericke, Thomas Heinze, Edda Tobiasch, Margit Schulze
Parent Title (English):International Journal of Molecular Sciences
Article Number:3565
Place of publication:Basel
Publishing Institution:Hochschule Bonn-Rhein-Sieg
Date of first publication:2019/07/21
Copyright:© 2019 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution.
Funding:This work was funded by the German Federal Ministry of Education and Research (BMBF) programs “IngenieurNachwuchs 2015” and “PersoImplant” (Grant No. 13FH019IX5), FHInvest (Grant No. 13FH158IN6), and BRSUGraduate Institute (scholarship for P.F.O.).
Keyword:agarose; biocomposite; bone tissue engineering; drug release; hydrogel; hydroxyapatite
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Institut für Technik, Ressourcenschonung und Energieeffizienz (TREE)
Projects:IngenieurNachwuchs 2015: PersoImplant - Personalisierte zellbesiedelte Implantate für Knochendefekte mit 'kritischer Größe' (DE/BMBF/03FH019IX5,13FH019IX5)
FHInvest 2016: Feldemissions-Elektronenmikroskop mit Computertomographie-System für Materialentwicklungen und Sicherheitstechnologien (DE/BMBF/13FH158IN6)
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Open access funding:Hochschule Bonn-Rhein-Sieg / Graduierteninstitut
Entry in this database:2019/07/25
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International