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Development and Evaluation of a Prototype Scratch Apparatus for Wound Assays Adjustable to Different Forces and Substrates

  • Scratch assays enable the study of the migration process of an injured adherent cell layer in vitro. An apparatus for the reproducible performance of scratch assays and cell harvesting has been developed that meets the requirements for reproducibility in tests as well as easy handling. The entirely autoclavable setup is divided into a sample translation and a scratching system. The translational system is compatible with standard culture dishes and can be modified to adapt to different cell culture systems, while the scratching system can be adjusted according to angle, normal force, shape, and material to adapt to specific questions and demanding substrates. As a result, a fully functional prototype can be presented. This system enables the creation of reproducible and clear scratch edges with a low scratch border roughness within a monolayer of cells. Moreover, the apparatus allows the collection of the migrated cells after scratching for further molecular biological investigations without the need for a second processing step. For comparison, the mechanical properties of manually performed scratch assays are evaluated.

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Document Type:Article
Author:Roman Grimmig, Patrick Babczyk, Philipp Gillemot, Klaus-Peter Schmitz, Margit Schulze, Edda Tobiasch
Parent Title (English):Applied Sciences
Article Number:4414
Number of pages:11
Place of publication:Basel
Publishing Institution:Hochschule Bonn-Rhein-Sieg
Date of first publication:2019/10/18
Copyright:© 2019 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Funding:This work was supported and financed by the Federal Ministry of Education and Research program “FHprofUnt” project “MeMoAthero” (grant number: 03FH012PB2).
Keyword:cell harvesting; cell migration; endothelial cells; prototype apparatus; scratch assay; wound healing assay
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Institut für Technik, Ressourcenschonung und Energieeffizienz (TREE)
Projects:IngenieurNachwuchs 2015: PersoImplant - Personalisierte zellbesiedelte Implantate für Knochendefekte mit 'kritischer Größe' (DE/BMBF/03FH019IX5,13FH019IX5)
MeMoAthero - FHprofUnt2012: Mechanismus und Modell der Atherosklerose: Entwicklung eines Medikamententestsystems für einen neuartigen Behandlungsansatz (DE/BMBF/03FH012PB2,13FH012PB2)
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Open access funding:Hochschule Bonn-Rhein-Sieg / Graduierteninstitut
Entry in this database:2019/10/23
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International