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NMR Spectroscopy as an Alternative Analytical Method for Biopolymers Without Chromophore: Example of Hyaluronic Acid in Dietary Supplements

  • To respond to the increasing demand for hyaluronic acid (HA) in dietary supplements (DSs) and nutricosmetics marketed for the treatment of osteoarthritis or moistening, it is essential to have an accurate and reliable method for its analysis in the final products. The study aimed to develop and validate alternative method for the quality control of HA in DSs using low-field (LF) and high-field (HF) nuclear magnetic resonance (NMR) spectroscopy at 80 MHz and 600 MHz, respectively. Moreover, chondroitin sulphate (CH), another active ingredient in DSs, can be simultaneously quantified. The 1H-NMR methods have been successfully validated in terms of limit of detection (LOD) and limit of quantitation (LOQ), which were found to be 0.1 mg/mL and 0.2 mg/mL (80 MHz) as well as 0.2 mg/mL and 0.6 mg/mL (600 MHz). Recovery rates were estimated to be between 92 and 120% on both spectrometers; precision including sample preparation was found to be 4.2% and 8.0% for 600 MHz and 80 MHz, respectively. Quantitative results obtained by HF and LF NMR were comparable for 16 DSs with varying matrix. HF NMR experiments at 70 ℃ serve as a simple and efficient quality control tool for HA and CH in multicomponent DSs. Benchtop NMR measurements, upon preceding acid hydrolysis, offer a cost-effective and cryogen-free alternative for analyzing DSs in the absence of CH and paramagnetic matrix components.

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Document Type:Article
Author:Fabienne M. Lang, Klaudia Adels, Bernd W. K. Diehl, Margit Schulze, Yulia B. Monakhova
Parent Title (English):Applied Magnetic Resonance
Date of first publication:2024/06/05
Copyright:© The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License.
Funding:Open Access funding enabled and organized by Projekt DEAL. This study was supported by University of Applied Sciences Aachen (internal project 10 403 430 04).
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Institut für Technik, Ressourcenschonung und Energieeffizienz (TREE)
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Entry in this database:2024/06/07
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International