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Extended beta distributions open the access to fast gating in bilayer experiments-assigning the voltage-dependent gating to the selectivity filter

  • Lipid bilayers provide many benefits for ion channel recordings, such as control of membrane composition and transport molecules. However, they suffer from high membrane capacitance limiting the bandwidth and impeding analysis of fast gating. This can be overcome by fitting the deviations of the open-channel noise from the baseline noise by extended beta distributions. We demonstrate this analysis step-by-step by applying it to the example of viral K+  channels (Kcv), from the choice of the gating model through the fitting process, validation of the results, and what kinds of results can be obtained. These voltage sensor-less channels show profoundly voltage-dependent gating with dwell times in the closed state of about 50 μs. Mutations assign it to the selectivity filter.

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Metadaten
Document Type:Article
Language:English
Author:Oliver Rauh, Ulf-Peter Hansen, Sebastian Mach, Andreas J. W. Hartel, Kenneth L. Shepard, Gerhard Thiel, Indra Schroeder
Parent Title (English):FEBS letters
Volume:591
Issue:23
Number of pages:11
First Page:3850
Last Page:3860
ISSN:0014-5793
DOI:https://doi.org/10.1002/1873-3468.12898
PMID:https://pubmed.ncbi.nlm.nih.gov/29106736
Date of first publication:2017/11/19
Keyword:amplitude histograms; mutational studies; rate constants; simulated time series; submillisecond and μs gating; viral K+ channels
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Entry in this database:2024/09/17