A Monoclonal Antibody Against Bovine Adiponectin

  • Adiponectin (AdipoQ) is an adipokine mainly secreted by white fatty tissue, playing a major role in energy homeostasis and insulin sensitivity. For cattle, AdipoQ data are largely limited to mRNA expression; to our knowledge, valid information about the AdipoQ protein in bovine tissues and body fluids is not available. Therefore, we have developed a monoclonal antibody against bovine AdipoQ. This study describes the preparation, application, and characterization of a monoclonal antibody for use in ELISA, Western blot, and histology. The antibody was developed by PEG fusion of the SP2/0 cell line with splenic B cells from AdipoQ immunized C57Bl/6 mice. Antibody-producing cells were identified by ELISA and specified by immunoblotting and immunostaining of bovine retroperitoneal adipose tissue. The novel antibody detects AdipoQ in histological samples, ELISA, and Western blots.

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Metadaten
Document Type:Article
Language:English
Author:Michael Raffelsieper, Birgit Mielenz, Susanne Häußler, Helga Sauerwein, Manfred Mielenz, Harald Illges
Parent Title (English):Hybridoma
Volume:31
Issue:6
First Page:465
Last Page:468
ISSN:1557-8348
DOI:https://doi.org/10.1089/hyb.2012.0054
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=23244328
Publisher:Mary Ann Liebert
Date of first publication:2012/12/17
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Institut für funktionale Gen-Analytik (IFGA)
Dewey Decimal Classification (DDC):6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Entry in this database:2015/04/02