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Non-Cytotoxic Agarose/Hydroxyapatite Composite Scaffolds for Drug Release

  • Healing of large bone defects requires implants or scaffolds that provide structural guidance for cell growth, differentiation, and vascularization. In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system. Hydroxyapatite (HA) was incorporated into the agarose gels in situ in various ratios by a simple procedure consisting of precipitation, cooling, washing, and drying. The resulting gels were characterized regarding composition, porosity, mechanical properties, and biocompatibility. A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers. Mechanical testing revealed elastic moduli of up to 2.8 MPa for lyophilized composites. MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds. Furthermore, scaffolds were loaded with model drug compounds for guided hMSC differentiation. Different release kinetic models were evaluated for adenosine 5′-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.

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Metadaten
Document Type:Article
Language:English
Author:Markus Witzler, Patrick Frank Ottensmeyer, Martin Gericke, Thomas Heinze, Edda Tobiasch, Margit Schulze
Parent Title (English):International Journal of Molecular Sciences
Volume:20
Issue:14
First Page:3565
ISSN:1422-0067
URN:urn:nbn:de:hbz:1044-opus-45564
DOI:https://doi.org/10.3390/ijms20143565
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=31330875
Publisher:MDPI
Place of publication:Basel
Publishing Institution:Hochschule Bonn-Rhein-Sieg
Date of first publication:2019/07/21
Note:
© 2019 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution.
Tag:agarose; biocomposite; bone tissue engineering; drug release; hydrogel; hydroxyapatite
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Open Access Funding:Hochschule Bonn-Rhein-Sieg H-BRS / Graduierteninstitut GI
Entry in this database:2019/07/25
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International