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In Vitro and In Vivo Antimalarial Activity Assays of Seeds from Balanites aegyptiaca: Compounds of the Extract Show Growth Inhibition and Activity against Plasmodial Aminopeptidase

  • Balanites aegyptiaca (Balanitaceae) is a widely grown desert plant with multiuse potential. In the present paper, a crude extract from B. aegyptiaca seeds equivalent to a ratio of 1 : 2000 seeds to the extract was screened for antiplasmodial activity. The determined IC(50) value for the chloroquine-susceptible Plasmodium falciparum NF54 strain was 68.26 μg/μL ± 3.5. Analysis of the extract by gas chromatography-mass spectrometry detected 6-phenyl-2(H)-1,2,4-triazin-5-one oxime, an inhibitor of the parasitic M18 Aspartyl Aminopeptidase as one of the compounds which is responsible for the in vitro antiplasmodial activity. The crude plant extract had a K(i) of 2.35 μg/μL and showed a dose-dependent response. After depletion of the compound, a significantly lower inhibition was determined with a K(i) of 4.8 μg/μL. Moreover, two phenolic compounds, that is, 2,6-di-tert-butyl-phenol and 2,4-di-tert-butyl-phenol, with determined IC(50) values of 50.29 μM ± 3 and 47.82 μM ± 2.5, respectively, were detected. These compounds may contribute to the in vitro antimalarial activity due to their antioxidative properties. In an in vivo experiment, treatment of BALB/c mice with the aqueous Balanite extract did not lead to eradication of the parasites, although a reduced parasitemia at day 12 p.i. was observed.

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Metadaten
Document Type:Article
Language:English
Author:Peter Kusch, Susanne Deininger, Sabine Specht, Rudeka Maniako, Stefanie Haubrich, Tanja Pommerening, Paul Kong Thoo Lin, Achim Hoerauf, Annette Kaiser
Parent Title (English):J Parasitol Res. (Journal of Parasitology Research)
Volume:2011
Pagenumber:9
First Page:368692
ISSN:2090-0031
URN:urn:nbn:de:hbz:1044-opus-8527
DOI:https://doi.org/10.1155/2011/368692
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=21687598
Publisher:Hindawi
Publishing Institution:Hochschule Bonn-Rhein-Sieg
Date of first publication:2011/05/25
Note:
Copyright © 2011 Peter Kusch et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Entry in this database:2015/04/02
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 3.0