DT-13 attenuates inflammation by inhibiting NLRP3-inflammasome related genes in RAW264.7 macrophages
- Plant derived saponins or other glycosides are widely used for their anti-inflammatory, antioxidant, and anti-viral properties in therapeutic medicine. In this study, we focus on understanding the function of the less known steroidal saponin from the roots of Liriope muscari L. H. Bailey – saponin C (also known as DT-13) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages in comparison to the well-known saponin ginsenoside Rk1 and anti-inflammatory drug dexamethasone. We proved that DT-13 reduces LPS-induced inflammation by inhibiting nitric oxide (NO) production, interleukin-6 (IL-6) release, cycloxygenase-2 (COX-2), tumour necrosis factor-alpha (TNF-α) gene expression, and nuclear factor kappa-B (NFκB) translocation into the nucleus. It also inhibits the inflammasome component NOD-like receptor family pyrin domain containing protein 3 (NLRP3) regulating the inflammasome activation. This was supported by the significant inhibition of caspase-1 and interleukin-1 beta (IL-1β) expression and release. This study demonstrates the anti-inflammatory effect of saponins on LPS-stimulated macrophages. For the first time, an in vitro study shows the attenuating effect of DT-13 on NLRP3-inflammasome activation. In comparison to the existing anti-inflammatory drug, dexamethasone, and triterpenoid saponin Rk1, DT-13 more efficiently inhibits inflammation in the applied cell culture model. Therefore, DT-13 may serve as a lead compound for the development of new more effective anti-inflammatory drugs with minimised side effects.
Document Type: | Article |
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Language: | English |
Author: | Shikha Raina, Emely Hübner, Esther Samuel, Gregor Nagel, Hendrik Fuchs |
Parent Title (English): | Biochemical and Biophysical Research Communications |
Volume: | 3 |
Issue: | 7 |
Article Number: | 149763 |
Number of pages: | 8 |
ISSN: | 0006-291X |
URN: | urn:nbn:de:hbz:1044-opus-82582 |
DOI: | https://doi.org/10.1016/j.bbrc.2024.149763 |
PMID: | https://pubmed.ncbi.nlm.nih.gov/38503169 |
Publisher: | Elsevier |
Publishing Institution: | Hochschule Bonn-Rhein-Sieg |
Date of first publication: | 2024/03/13 |
Copyright: | © 2024 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license. |
Funding: | This research was supported by the Deutsche Forschungsgemeinschaft (DFG) (Grant no: FU 408/11-1). |
Keyword: | Anti-inflammatory effects; NFκB pathway; NLRP3 inflammasome; Steroidal saponin |
Departments, institutes and facilities: | Fachbereich Angewandte Naturwissenschaften |
Dewey Decimal Classification (DDC): | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 572 Biochemie |
Entry in this database: | 2024/04/11 |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |