Simultaneous determination of caffeine in cola drinks and other beverages by reversed-phase HPTLC and reversed-phase HPLC
- A simple reversed-phase high-performance thin-layer chromatographic (HPTLC) method for the identification and quantitative determination of caffeine in Coca-Cola is described. The chromatographic conditions of the HPTLC method are further adapted by developing the HPLC method for identification and determination of caffeine in cola drinks and other caffeine-containing beverages. The results of the quantitative determinations of caffeine in Coca-Cola obtained by using both HPTLC and HPLC methods were 96.1 ± 1.1 mg L–1 and 95.4 ± 0.2 mg L–1, respectively. The experimental objective for the students is to determine the unknown amount of caffeine in a complex matrix by two different chromatographic techniques. The pedagogical objective is to make students familiar with these well-established techniques. Furthermore, they learn about the usefulness, difficulties, and limitations of comparative analytical studies. The two-part experiment has been developed for second-year chemistry and biology undergraduate students and is performed in the instrumental analysis course in two three-hour laboratory sessions.
| Document Type: | Article |
|---|---|
| Language: | English |
| Author: | Peter Kusch, Gerd Knupp |
| Parent Title (English): | Chem. Educ. (Chemical Educator) |
| Volume: | 8 |
| Issue: | 3 |
| First Page: | 201 |
| Last Page: | 205 |
| ISSN: | 1430-4171 |
| URL: | http://chemeducator.org/bibs/0008003/830201pk.htm |
| Date of first publication: | 2003/04/11 |
| Tag: | Caffeine-containing drinks; HPLC; HPTLC; Instrumental analysis; Laboratories and Demonstrations |
| Departments, institutes and facilities: | Fachbereich Angewandte Naturwissenschaften |
| Dewey Decimal Classification (DDC): | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Entry in this database: | 2015/04/02 |
