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Simultaneous determination of caffeine in cola drinks and other beverages by reversed-phase HPTLC and reversed-phase HPLC

  • A simple reversed-phase high-performance thin-layer chromatographic (HPTLC) method for the identification and quantitative determination of caffeine in Coca-Cola is described. The chromatographic conditions of the HPTLC method are further adapted by developing the HPLC method for identification and determination of caffeine in cola drinks and other caffeine-containing beverages. The results of the quantitative determinations of caffeine in Coca-Cola obtained by using both HPTLC and HPLC methods were 96.1 ± 1.1 mg L–1 and 95.4 ± 0.2 mg L–1, respectively. The experimental objective for the students is to determine the unknown amount of caffeine in a complex matrix by two different chromatographic techniques. The pedagogical objective is to make students familiar with these well-established techniques. Furthermore, they learn about the usefulness, difficulties, and limitations of comparative analytical studies. The two-part experiment has been developed for second-year chemistry and biology undergraduate students and is performed in the instrumental analysis course in two three-hour laboratory sessions.

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Document Type:Article
Author:Peter Kusch, Gerd Knupp
Parent Title (English):Chem. Educ. (Chemical Educator)
First Page:201
Last Page:205
Date of first publication:2003/04/11
Keyword:Caffeine-containing drinks; HPLC; HPTLC; Instrumental analysis; Laboratories and Demonstrations
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Entry in this database:2015/04/02