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Spatial patterning of nanofibrous collagen scaffolds modulates fibroblast morphology

  • Current knowledge about cell-biomaterial interactions is often based on 2D cell culture systems like protein-coated glass slides. However, such smooth surfaces cannot mimic the nanofibrous environment of the native extracellular matrix (ECM). It is therefore a major challenge to transfer the results from 2D surfaces to 3D protein scaffolds with biomimetic nanofiber architecture. To understand the influence of different protein topographies on the cell response we introduce a new process to fabricate binary collagen scaffolds of variable thickness with spatially controlled regions of nanofibrous and smooth topography. We used pH-induced self-assembly to prepare collagen nanofibers with diameters between 130 and 150 nm on glass surfaces, which were partly covered with a polymer mask. After cross-linking with glutaraldehyde, smooth collagen films were prepared on the remaining glass regions. Atomic force microscopy revealed a much lower surface roughness of smooth collagen compared to nanofibers.

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Document Type:Article
Author:Naiana Suter, Sophie Stebel, Carmela Rianna, Manfred Radmacher, Dorothea Brüggemann
Parent Title (English):Biofabrication
First Page:015007
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=33135668
Publisher:IOP Publishing
Date of first publication:2020/10/23
© Copyright 2020 IOP Publishing.
The authors gratefully acknowledge funding by the Emmy Noether program of the German Research Council under Grant No. BR 5043/1-1.
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Entry in this database:2020/10/07