Volltext-Downloads (blau) und Frontdoor-Views (grau)

Flotillins Are Involved in the Polarization of Primitive and Mature Hematopoietic Cells

  • Background: Migration of mature and immature leukocytes in response to chemokines is not only essential during inflammation and host defense, but also during development of the hematopoietic system. Many molecules implicated in migratory polarity show uniform cellular distribution under non-activated conditions, but acquire a polarized localization upon exposure to migratory cues. Methodology/Principal Findings: Here, we present evidence that raft-associated endocytic proteins (flotillins) are preassembled in lymphoid, myeloid and primitive hematopoietic cells and accumulate in the uropod during migration. Furthermore, flotillins display a polarized distribution during immunological synapse formation. Employing the membrane lipid-order sensitive probe Laurdan, we show that flotillin accumulation in the immunological synapse is concomittant with membrane ordering in these regions. Conclusions: Together with the observation that flotillin polarization does not occur in other polarized cell types such as polarized epithelial cells, our results suggest a specific role for flotillins in hematopoietic cell polarization. Based on our results, we propose that in hematopoietic cells, flotillins provide intrinsic cues that govern segregation of certain microdomain-associated molecules during immune cell polarization.

Download full text files

Export metadata

Additional Services

Search Google Scholar Check availability


Show usage statistics
Document Type:Article
Author:Lawrence Rajendran, Julia Beckmann, Astrid Magenau, Eva-Maria Boneberg, Katharina Gaus, Antonella Viola, Bernd Giebel, Harald Illges
Parent Title (English):PLoS ONE
Article Number:e8290
First Page:1
Last Page:12
Publisher:Public Library of Science (PLoS)
Place of publication:San Francisco, CA, USA
Publishing Institution:Hochschule Bonn-Rhein-Sieg
Date of first publication:2009/12/22
Copyright:(c) 2009 Rajendran et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License.
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Institut für funktionale Gen-Analytik (IFGA)
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Entry in this database:2018/06/06
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 3.0