Hydrostatic pressure induces apoptosis in the human leukaemic T-cell line Jurkat via the mitochondrial pathway
- We investigated the effect of pressure levels ranging from 80 to 500 bar on the proliferative capacity and viability of Jurkat leukaemic T cells. Pressurization at 360 bar induced apoptotic cell death as shown by apoptotic morphology after Hoechst staining, DNA fragmentation in the TdT-mediated dUTP nick end labelling-assay and cleavage of several caspase substrates. Cell death could be prevented by the general caspase inhibitor zVAD-fmk. Breakdown of the mitochondrial membrane potential and the release of cytochrome c provided strong evidence for an involvement of the mitochondrial pathway, whereas a central role of the death receptor pathway was excluded because caspase-8 was not significantly activated. Pressure incubation led to calcium influx after 5 min, and we hypothesize that calcium influx could be the primary trigger for pressure-induced apoptosis.
| Document Type: | Article |
|---|---|
| Language: | English |
| Author: | M. V. Klett, E-M Boneberg, K. Trenz, R. Knippers, H. Illges |
| Parent Title (English): | Scand J Immunol. (Scandinavian Journal of Immunology) |
| Volume: | 60 |
| Issue: | 4 |
| First Page: | 403 |
| Last Page: | 411 |
| ISSN: | 0300-9475 |
| DOI: | https://doi.org/10.1111/j.0300-9475.2004.01496.x |
| PMID: | https://pubmed.ncbi.nlm.nih.gov/15379865 |
| Publisher: | Blackwell Publishing |
| Date of first publication: | 2004/09/20 |
| Departments, institutes and facilities: | Institut für funktionale Gen-Analytik (IFGA) |
| Dewey Decimal Classification (DDC): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Entry in this database: | 2018/07/25 |
