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Examination of the Quality of Particulate and Filtered Mandibular Bone Chips for Oral Implants: An In Vitro Study

  • (1) Background: Autologous bone is supposed to contain vital cells that might improve the osseointegration of dental implants. The aim of this study was to investigate particulate and filtered bone chips collected during oral surgery intervention with respect to their osteogenic potential and the extent of microbial contamination to evaluate its usefulness for jawbone reconstruction prior to implant placement. (2) Methods: Cortical and cortical-cancellous bone chip samples of 84 patients were collected. The stem cell character of outgrowing cells was characterized by expression of CD73, CD90 and CD105, followed by osteogenic differentiation. The degree of bacterial contamination was determined by Gram staining, catalase and oxidase tests and tests to evaluate the genera of the found bacteria (3) Results: Pre-surgical antibiotic treatment of the patients significantly increased viability of the collected bone chip cells. No significant difference in plasticity was observed between cells isolated from the cortical and cortical-cancellous bone chip samples. Thus, both types of bone tissue can be used for jawbone reconstruction. The osteogenic differentiation was independent of the quantity and quality of the detected microorganisms, which comprise the most common bacteria in the oral cavity. (4) Discussion: This study shows that the quality of bone chip-derived stem cells is independent of the donor site and the extent of present common microorganisms, highlighting autologous bone tissue, assessable without additional surgical intervention for the patient, as a useful material for dental implantology.

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Document Type:Article
Author:Patrick Babczyk, Martin Winter, Claudia Kleinfeld, Andreas Pansky, Christina Oligschleger, Edda Tobiasch
Parent Title (English):Applied Sciences
Article Number:2031
Number of pages:15
Place of publication:Basel
Publishing Institution:Hochschule Bonn-Rhein-Sieg
Date of first publication:2022/02/16
Copyright:© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Funding:This research was funded by AdiPaD, BMBF-AiF, FKZ: 1720X06; Geräteprogramm, Fachhochschulen 2008 and 2009; HybridKEM, FKZ: 13FH569IX6.
Keyword:antibiotic prophylaxis; autologous bone graft; cell viability; dental implant; mesenchymal stem cells; microbial contamination; osteogenic potential
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Projects:IngenieurNachwuchs 2016: Hybrid-KEM - Entwicklung neuer Hybride auf Phosphat/Silicat/Agarose-Basis als KnochenErsatzMaterial mit Release-Funktion für Bisphosphonat-Wirkstoffe (DE/BMBF/13FH569IX6)
Untersuchung des Einflusses ausdifferenzierender Adipozyten bei der Pathogenese des Diabetes mellitus Typ 2 (AdiPaD) (DE/BMBF/1720X06;1320X06)
Dewey Decimal Classification (DDC):6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Open access funding:Hochschule Bonn-Rhein-Sieg / Graduierteninstitut
Entry in this database:2022/02/25
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International