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Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins

  • This research studies in detail four different assays, namely DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), FRAP (ferric ion reducing antioxidant potential) and FC (Folin-Ciocalteu), to determine the antioxidant capacity of standard substances as well as 50 organosolv lignins, and two kraft lignins. The coefficient of variation was determined for each method and was lowest for ABTS and highest for DPPH. The best correlation was found for FRAP and FC, which both rely on a single electron transfer mechanism. A good correlation between ABTS, FRAP and FC, respectively, could be observed, even though ABTS relies on a more complex reaction mechanism. The DPPH assay merely correlates with the others, implying that it reflects different antioxidative attributes due to a different reaction mechanism. Lignins obtained from paulownia and silphium have been investigated for the first time regarding their antioxidant capacity. Paulownia lignin is in the same range as beech wood lignin, while silphium lignin resembles wheat straw lignin. Miscanthus lignin is an exception from the grass lignins and possesses a significantly higher antioxidant capacity. All lignins possess a good antioxidant capacity and thus are promising candidates for various applications, e. g. as additives in food packaging or for biomedical purposes.

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Document Type:Article
Author:Jessica Rumpf, René Burger, Margit Schulze
Parent Title (English):International Journal of Biological Macromolecules
Article Number:123470
Number of pages:9
Publishing Institution:Hochschule Bonn-Rhein-Sieg
Date of first publication:2023/02/02
Copyright:© 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.
Funding:This study was supported by the BMBF (grant no. 13FH102PX8) and EFRE/NRW (grant no. EFRE 0500035). J.R. gratefully acknowledges a scholarship given by the Graduate Institute of the Bonn-Rhein-Sieg University of Applied Sciences.
Keyword:Antioxidant capacity; Assay reproducibility; Lignin
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Institut für Technik, Ressourcenschonung und Energieeffizienz (TREE)
Projects:BiopolymerModell - FHprofUnt 2018: Neuer Zugang zur Analyse von Biopolymeren: Kombination experimenteller Methoden mit chemometrischer Modellierung (DE/BMBF/13FH102PX8)
Biobasierte Produkte (EFRE/0500035)
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Open access funding:Hochschule Bonn-Rhein-Sieg / Graduierteninstitut
Hochschule Bonn-Rhein-Sieg / Publikationsfonds / 2022 ff
Entry in this database:2023/02/09
Licence (German):License LogoCreative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International