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Dried serum spots on pre-punched filter paper discs are ready-to-use storage and shipping devices for blood-borne antigens and antibodies

  • Dried serum spots that are well prepared can be attractive alternatives to frozen serum samples for shelving specimens in a medical or research center's biobank and mailing freshly prepared serum to specialized laboratories. During the pre-analytical phase, complications can arise which are often challenging to identify or are entirely overlooked. These complications can lead to reproducibility issues, which can be avoided in serum protein analysis by implementing optimized storage and transfer procedures. With a method that ensures accurate loading of filter paper discs with donor or patient serum, a gap in dried serum spot preparation and subsequent serum analysis shall be filled. Pre-punched filter paper discs with a 3 mm diameter are loaded within seconds in a highly reproducible fashion (approximately 10% standard deviation) when fully submerged in 10 μl of serum, named the "Submerge and Dry" protocol. Such prepared dried serum spots can store several hundred micrograms of proteins and other serum components. Serum-borne antigens and antibodies are reproducibly released in 20 μl elution buffer in high yields (approximately 90%). Dried serum spot-stored and eluted antigens kept their epitopes and antibodies their antigen binding abilities as was assessed by SDS-PAGE, 2D gel electrophoresis-based proteomics, and Western blot analysis, suggesting pre-punched filter paper discs as handy solution for serological tests.

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Document Type:Article
Author:Kira Billinger, Charles A. Okai, Manuela Russ, Cornelia Koy, Claudia Röwer, Kwabena F. M. Opuni, Harald Illges, Ulrich Pecks, Michael O. Glocker
Parent Title (English):Journal of Immunological Methods
Article Number:113519
Number of pages:8
Publishing Institution:Hochschule Bonn-Rhein-Sieg
Date of first publication:2023/07/06
Copyright:© 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license
Keyword:Antibody analysis; Antigen analysis; Dried serum spots; Patient serum; Pre-punched filter paper discs; Proteome analysis; Submerge and dry protocol
Departments, institutes and facilities:Fachbereich Wirtschaftswissenschaften
Institut für funktionale Gen-Analytik (IFGA)
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Entry in this database:2023/07/11
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International