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Seasonality Is the Main Determinant of Microbial Diversity Associated to Snow/Ice around Concordia Station on the Antarctic Polar Plateau

  • The French–Italian Concordia Research Station, situated on the Antarctic Polar Plateau at an elevation of 3233 m above sea level, offers a unique opportunity to study the presence and variation of microbes introduced by abiotic or biotic vectors and, consequently, appraise the amplitude of human impact in such a pristine environment. This research built upon a previous work, which explored microbial diversity in the surface snow surrounding the Concordia Research Station. While that study successfully characterized the bacterial assemblage, detecting fungal diversity was hampered by the low DNA content. To address this knowledge gap, in the present study, we optimized the sampling by increasing ice/snow collected to leverage the final DNA yield. The V4 variable region of the 16S rDNA and Internal Transcribed Spacer (ITS1) rDNA was used to evaluate bacterial and fungal diversity. From the sequencing, we obtained 3,352,661 and 4,433,595 reads clustered in 930 and 3182 amplicon sequence variants (ASVs) for fungi and bacteria, respectively. Amplicon sequencing revealed a predominance of Basidiomycota (49%) and Ascomycota (42%) in the fungal component; Bacteroidota (65.8%) is the main representative among the bacterial phyla. Basidiomycetes are almost exclusively represented by yeast-like fungi. Our findings provide the first comprehensive overview of both fungal and bacterial diversity in the Antarctic Polar Plateau’s surface snow/ice near Concordia Station and to identify seasonality as the main driver of microbial diversity; we also detected the most sensitive microorganisms to these factors, which could serve as indicators of human impact in this pristine environment and aid in planetary protection for future exploration missions.

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Metadaten
Document Type:Article
Language:English
Author:Gerardo A. Stoppiello, Claudia Coleine, Ralf Moeller, Caterina Ripa, Daniela Billi, Laura Selbmann
Parent Title (English):Biology
Volume:12
Issue:9
Article Number:1193
Number of pages:13
ISSN:2079-7737
URN:urn:nbn:de:hbz:1044-opus-75251
DOI:https://doi.org/10.3390/biology12091193
PMID:https://pubmed.ncbi.nlm.nih.gov/37759592
Publisher:MDPI
Place of publication:Basel
Publishing Institution:Hochschule Bonn-Rhein-Sieg
Date of first publication:2023/08/31
Copyright:© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Funding:This research was supported by the National Antarctic Research Program (MIcrobial Diversity within the Vicinity of the Concordia Antarctic Station _MIDAS project grant PNRA 2016_00101 to D.B. and L.S.). N.J.U. and R.M. were supported by the DLR grant FuE-Projekt “ISS LIFE” (Programm RF-FuW, TP 475). The BacFinder project was supported by a Europlanet 2020 RI grant from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 654208. C.C. is supported by the European Commission under the Marie Sklodowska-Curie Grant Agreement No. 702057 (DRYLIFE).
Keyword:Antarctic Polar Plateau; amplicon sequencing; bacteria; extraterrestrial analogue; extremophiles; fungi; life detection
Departments, institutes and facilities:Fachbereich Angewandte Naturwissenschaften
Institut für funktionale Gen-Analytik (IFGA)
Dewey Decimal Classification (DDC):5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 579 Mikroorganismen, Pilze, Algen
Entry in this database:2023/09/19
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International