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Background: Human mesenchymal stem cells (hMSCs) have shown their multipotential including differentiating towards endothelial and smooth muscle cell lineages, which triggers a new interest for using hMSCs as a putative source for cardiovascular regenerative medicine. Our recent publication has shown for the first time that purinergic 2 receptors are key players during hMSC differentiation towards adipocytes and osteoblasts. Purinergic 2 receptors play an important role in cardiovascular function when they bind to extracellular nucleotides. In this study, the possible functional role of purinergic 2 receptors during MSC endothelial and smooth muscle differentiation was investigated. Methods and Results: Human MSCs were isolated from liposuction materials. Then, endothelial and smooth muscle-like cells were differentiated and characterized by specific markers via Reverse Transcriptase-PCR (RT-PCR), Western blot and immunochemical stainings. Interestingly, some purinergic 2 receptor subtypes were found to be differently regulated during these specific lineage commitments: P2Y4 and P2Y14 were involved in the early stage commitment while P2Y1 was the key player in controlling MSC differentiation towards either endothelial or smooth muscle cells. The administration of natural and artificial purinergic 2 receptor agonists and antagonists had a direct influence on these differentiations. Moreover, a feedback loop via exogenous extracellular nucleotides on these particular differentiations was shown by apyrase digest. Conclusions: Purinergic 2 receptors play a crucial role during the differentiation towards endothelial and smooth muscle cell lineages. Some highly selective and potent artificial purinergic 2 ligands can control hMSC differentiation, which might improve the use of adult stem cells in cardiovascular tissue engineering in the future.
Graph drawing with spring embedders employs a V x V computation phase over the graph's vertex set to compute repulsive forces. Here, the efficacy of forces diminishes with distance: a vertex can effectively only influence other vertices in a certain radius around its position. Therefore, the algorithm lends itself to an implementation using search data structures to reduce the runtime complexity. NVIDIA RT cores implement hierarchical tree traversal in hardware. We show how to map the problem of finding graph layouts with force-directed methods to a ray tracing problem that can subsequently be implemented with dedicated ray tracing hardware. With that, we observe speedups of 4x to 13x over a CUDA software implementation.
Extremophiles are optimal models in experimentally addressing questions about the effects of cosmic radiation on biological systems. The resistance to high charge energy (HZE) particles, and helium (He) ions and iron (Fe) ions (LET at 2.2 and 200 keV/µm, respectively, until 1000 Gy), of spores from two thermophiles, Bacillushorneckiae SBP3 and Bacilluslicheniformis T14, and two psychrotolerants, Bacillus sp. A34 and A43, was investigated. Spores survived He irradiation better, whereas they were more sensitive to Fe irradiation (until 500 Gy), with spores from thermophiles being more resistant to irradiations than psychrotolerants. The survived spores showed different germination kinetics, depending on the type/dose of irradiation and the germinant used. After exposure to He 1000 Gy, D-glucose increased the lag time of thermophilic spores and induced germination of psychrotolerants, whereas L-alanine and L-valine increased the germination efficiency, except alanine for A43. FTIR spectra showed important modifications to the structural components of spores after Fe irradiation at 250 Gy, which could explain the block in spore germination, whereas minor changes were observed after He radiation that could be related to the increased permeability of the inner membranes and alterations of receptor complex structures. Our results give new insights on HZE resistance of extremophiles that are useful in different contexts, including astrobiology.
Der Beitrag untersucht, wie ein Präsenzlabor durch ein Remote-Labor ergänzt undersetzt werden kann. Dazu wird das Laborpraktikum Digitaltechnik der Hochschule Bonn-Rhein-Sieg betrachtet, bei dem ein Remote-Labor Flexibilität bei der Versuchsdurchführung bietet und Versuche ermöglicht, die allein mit dem Präsenzlabor nicht möglich wären. Neben der Ergänzung der Präsenzversuche können Studie-rende das Praktikum auch komplett im Remote-Labor durchführen. Durch klare Anforderungen an die Erteilung eines Testats ist dies sowohl für sie als auch für Lehrende praktikabel zu handhaben. Rückmeldungen der Studierenden und Nutzungszahlen belegen die Akzeptanz des Remote-Labors. Dabei zeigt sich, dass die Studierenden sehr heterogen mit dem Remote-Labor umgehen: Einige von ihnen nutzen das Remote-Labor als zusätzliche Praktikumszeit für Versuche die auch im Präsenzlabor möglich wären; andere nutzen es als Erweiterung der Praktikumsmöglichkeit für Versuche, die nur im Remote-Labor möglich sind und wieder andere arbeiten intensiv im Remote-Labor und reichen auch das Praktikumsprotokoll elektronisch ein. Für Lehrende besteht über das Protokoll und die Auswertung der Nutzungsdaten ausreichende Sicherheit, um aktive Beteiligung am Praktikum zu testieren.
Usability und User Experience (UX) haben als Design-Aspekte in der Produktentwicklung zunehmend an Bedeutung gewonnen. Daher ist es sinnvoll, die organisationale Kompetenz zur Entwicklung von Produkten mit einer positiven UX zu stärken. Veränderungen in Organisationen sind jedoch mit großem Aufwand verbunden. Deshalb müssen Organisationen entscheiden, welche Aktivitäten zur Veränderung der eigenen Kompetenz durchgeführt werden sollen und welche nicht. Die bisherige Forschung hat sich weitgehend auf die Anwendbarkeit bestimmter Methoden im Projekt- und Produktkontext konzentriert. Um geeignete Aktivitäten zur Verbesserung der organisationalen UX-Kompetenz zu identifizieren, wurden 17 UX-Professionals befragt. Diese UX-Professionals haben mindestens zehn Jahre Erfahrung durch die Arbeit in mehreren Unternehmen und durch die Übernahme einer Führungsrolle im Bereich UX gesammelt. Aus diesen Interviews wurden 13 mögliche Maßnahmen zur Steigerung der UX-Kompetenz von Organisationen abgeleitet. Dazu gehören beispielsweise die Erhöhung der Kompetenz einzelner Mitarbeiter, das Teilen von UX-Erfolgsgeschichten oder das Ermöglichen von User Research.
This work thoroughly investigates a semi-Lagrangian lattice Boltzmann (SLLBM) solver for compressible flows. In contrast to other LBM for compressible flows, the vertices are organized in cells, and interpolation polynomials up to fourth order are used to attain the off-vertex distribution function values. Differing from the recently introduced Particles on Demand (PoD) method , the method operates in a static, non-moving reference frame. Yet the SLLBM in the present formulation grants supersonic flows and exhibits a high degree of Galilean invariance. The SLLBM solver allows for an independent time step size due to the integration along characteristics and for the use of unusual velocity sets, like the D2Q25, which is constructed by the roots of the fifth-order Hermite polynomial. The properties of the present model are shown in diverse example simulations of a two-dimensional Taylor-Green vortex, a Sod shock tube, a two-dimensional Riemann problem and a shock-vortex interaction. It is shown that the cell-based interpolation and the use of Gauss-Lobatto-Chebyshev support points allow for spatially high-order solutions and minimize the mass loss caused by the interpolation. Transformed grids in the shock-vortex interaction show the general applicability to non-uniform grids.
Turbulent compressible flows are traditionally simulated using explicit Eulerian time integration applied to the Navier-Stokes equations. However, the associated Courant-Friedrichs-Lewy condition severely restricts the maximum time step size. Exploiting the Lagrangian nature of the Boltzmann equation's material derivative, we now introduce a feasible three-dimensional semi-Lagrangian lattice Boltzmann method (SLLBM), which elegantly circumvents this restriction. Previous lattice Boltzmann methods for compressible flows were mostly restricted to two dimensions due to the enormous number of discrete velocities needed in three dimensions. In contrast, this Rapid Communication demonstrates how cubature rules enhance the SLLBM to yield a three-dimensional velocity set with only 45 discrete velocities. Based on simulations of a compressible Taylor-Green vortex we show that the new method accurately captures shocks or shocklets as well as turbulence in 3D without utilizing additional filtering or stabilizing techniques, even when the time step sizes are up to two orders of magnitude larger compared to simulations in the literature. Our new method therefore enables researchers for the first time to study compressible turbulent flows by a fully explicit scheme, whose range of admissible time step sizes is only dictated by physics, while being decoupled from the spatial discretization.
This paper introduces FaceHaptics, a novel haptic display based on a robot arm attached to a head-mounted virtual reality display. It provides localized, multi-directional and movable haptic cues in the form of wind, warmth, moving and single-point touch events and water spray to dedicated parts of the face not covered by the head-mounted display.The easily extensible system, however, can principally mount any type of compact haptic actuator or object. User study 1 showed that users appreciate the directional resolution of cues, and can judge wind direction well, especially when they move their head and wind direction is adjusted dynamically to compensate for head rotations. Study 2 showed that adding FaceHaptics cues to a VR walkthrough can significantly improve user experience, presence, and emotional responses.
Risk-based Authentication (RBA) is an adaptive security measure that improves the security of password-based authentication by protecting against credential stuffing, password guessing, or phishing attacks. RBA monitors extra features during login and requests for an additional authentication step if the observed feature values deviate from the usual ones in the login history. In state-of-the-art RBA re-authentication deployments, users receive an email with a numerical code in its body, which must be entered on the online service. Although this procedure has a major impact on RBA's time exposure and usability, these aspects were not studied so far.
We introduce two RBA re-authentication variants supplementing the de facto standard with a link-based and another code-based approach. Then, we present the results of a between-group study (N=592) to evaluate these three approaches. Our observations show with significant results that there is potential to speed up the RBA re-authentication process without reducing neither its security properties nor its security perception. The link-based re-authentication via "magic links", however, makes users significantly more anxious than the code-based approaches when perceived for the first time. Our evaluations underline the fact that RBA re-authentication is not a uniform procedure. We summarize our findings and provide recommendations.
Risk-based Authentication (RBA) is an adaptive security measure to strengthen password-based authentication. RBA monitors additional features during login, and when observed feature values differ significantly from previously seen ones, users have to provide additional authentication factors such as a verification code. RBA has the potential to offer more usable authentication, but the usability and the security perceptions of RBA are not studied well.
We present the results of a between-group lab study (n=65) to evaluate usability and security perceptions of two RBA variants, one 2FA variant, and password-only authentication. Our study shows with significant results that RBA is considered to be more usable than the studied 2FA variants, while it is perceived as more secure than password-only authentication in general and comparably secure to 2FA in a variety of application types. We also observed RBA usability problems and provide recommendations for mitigation. Our contribution provides a first deeper understanding of the users' perception of RBA and helps to improve RBA implementations for a broader user acceptance.
Risikobasierte Authentifizierung (RBA) ist eine adaptive Sicherheitsmaßnahme zur Stärkung passwortbasierter Authentifizierung. Sie zeichnet Merkmale während des Logins auf und fordert zusätzliche Authentifizierung an, wenn sich Ausprägungen dieser Merkmale signifikant von den bisher bekannten unterscheiden. RBA bietet das Potenzial für gebrauchstauglichere Sicherheit. Bisher jedoch wurde RBA noch nicht ausreichend im Bezug auf Usability, Sicherheit und Privatsphäre untersucht. Dieser Extended Abstract legt das geplante Dissertationsvorhaben zur Erforschung von RBA dar. Innerhalb des Vorhabens konnte bereits eine Grundlagenstudie und eine darauf aufbauende Laborstudie durchgeführt werden. Wir präsentieren erste Ergebnisse dieser Studien und geben einen Ausblick auf weitere Schritte.
Studi ini bertujuan untuk memvalidasi perangkat penilaian efikasi diri yang berkaitan dengan kesehatan kerja yang dikembangkan pada tahap studi pendahuluan. Skala Efikasi Diri untuk Kesehatan Kerja (SEDKK) berlandaskan konsep efikasi diri pada teori kognitif sosial yang mengukur empat faktor yang berpengaruh pada kesehatan setiap individu yang bekerja, seperti: perilaku makan dan minum, tidur, keamanan dan kesehatan kerja, serta kegiatan pemulihan dari stres bekerja. Hasil analisis faktor eksploratori menunjukan bahwa ada empat faktor yang terefleksikan dari butir-butir SEDKK. Validitas konstruk SEDKK dapat dibuktikan dengan korelasi positif antara SEDKK dan skala Efikasi Diri Umum yang sangat signifikan. Pengujian validitas kriteria dapat ditelusuri melalui efek SEDKK terhadap kondisi kesehatan umum, kepuasan akan kesehatan pribadi, keseimbangan kehidupan kerja/KKK (work life balance), perilaku sehat, dan perilaku berisiko. Namun demikian, asumsi mengenai reliabilitas tes berulang (test-retest) pada penelitian ini ditolak. Implikasi dan saran-saran untuk penelitian selanjutnya didiskusikan pada artikel ini.