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Reggie-1/flotillin-2 is a plasma membrane-associated cytoplasmic protein, which defines non-caveolar raft microdomains. Reggie-1/flotillin-2 is enriched in detergent insoluble (TX100) membrane fractions (DIG), co-localizes with activated GPI-linked proteins and the fyn-kinase in neurons and T cells, and thus apparently participates in the assembly of protein complexes essential for signal transduction. In T cells activated by crosslinking the GPI-linked protein Thy-1 or by crosslinking the ganglioside GM1, reggie-1/flotillin-2 co-localizes with the T cell receptor. To determine whether reggie-1/flotillin-2 is also expressed in B cells, primary B cells from human blood and cell lines representing the developmental stages of pro, pre, mature and plasma B cells were analyzed by Western blotting, RT-PCR and immunofluorescence. Here, we show that reggie-1/flotillin-2 is expressed throughout B cell development, as well as in primary B cells, purified by cell sorting. On non-activated mature B cell Raji cell line we found reggie-1/flotillin-2 are exclusively in the detergent (TX100) insoluble membrane fractions that are staining positive for the raft marker GM1. Immunofluorescence microscopy showed that reggie-1/flotillin-2 is localized at the plasma membrane and marks intracellular spots in PBMCs. Confocal co-localization studies showed that reggie-1/flotillin-2 is associated with the plasma membrane, and the centrosomes (microtubule organizing centers) in these PBMCs. Comparison of reggie-1/flotillin-2 cDNA sequences with the genomic sequence database allowed us to determine the exon/intron structures in mouse and human. The gene organizations are highly conserved suggesting an important function of reggie-1/flotillin-2. Since reggie/flotillin proteins co-cluster with the T cell receptor and fyn kinases upon T cell stimulation, our findings of reggie-1/flotillin-2 in B cells suggest a similar role in B cell function.
Impaired up-regulation of CD86 in B cells of "type A" common variable immunodeficiency patients
(2000)
Daryoush Daniel Vaziri illustrates that the use of mixed methods designs may support the induction of more subtle and complete theories about older adults’ use of technologies for the support of active and healthy aging. The results show that older adults’ social contexts and environments considerably affect their perspectives, practices and attitudes with respect to health, quality of life, well-being and technology use for active and healthy aging support. Results were collected with older adults aged 60+ as well as relevant secondary stakeholders like caregivers, policy makers or health insurance companies.
Large, high-resolution displays are highly suitable for creation of digital environments for co-located collaborative task solving. Yet, placing multiple users in a shared environment may increase the risk of interferences, thus causing mental discomfort and decreasing efficiency of the team. To mitigate interferences coordination strategies and techniques were introduced. However, in a mixed-focus collaboration scenarios users switch now and again between loosely and tightly collaboration, therefore different coordination techniques might be required depending on the current collaboration state of team members. For that, systems have to be able to recognize collaboration states as well as transitions between them to ensure a proper adjustment of the coordination strategy. Previous studies on group behavior during collaboration in front of large displays investigated solely collaborative coupling states, not transitions between them though. To address this gap, we conducted a study with 12 participant dyads in front of a tiled display and let them solve two tasks in two different conditions (focus and overview). We looked into group dynamics and categorized transitions by means of changes in proximity, verbal communication, visual attention, visual interface, and gestures. The findings can be valuable for user interface design and development of group behavior models.
Urban food systems consist of many stakeholders with different perspectives, different interests and different governance tools. This study aimed at developing potential future scenarios for the food system of Cologne by analysing the system with a Delphi approach. In our research-design, the suitability of the Delphi-method was evaluated not only as a tool for future modelling and scenario design, but also as a communication tool among the group of participants on a multi-stakeholder-platform. As a case study, the Food Policy Council of Cologne, Germany was used. Cologne can be seen as a forerunner among German cities in the development of a new urban food policy. Some of the successful steps to re-envisioning food as an urban system include joining the Milan Urban Food Policy Pact, the decision of the City Council to become an edible city and the establishment of a Food Policy Council. For the study it was important to capture participants’ visions of a common goal regarding the governance of the urban food system and also to identify mental ‘silos’. It was obvious that the municipality of Cologne together with the Food Policy Council made great efforts towards participatory processes to build a vision for a sustainable and regional food supply. However, many stakeholder-groups in the process still work exclusively among themselves and do not actively practice the confrontation with the viewpoints of other relevant groups. This supports the maintenance of ‘silos’ and leaves little room for face-to-face discussions. Therefore, the primary aim of this study is to explore key components of food provisioning in the future for Cologne while confronting all stakeholders (municipal administration and politicians, farmers and food activists) with the perspectives of all group members. We used a multi-stakeholder Delphi approach with 19 panellists to find out essential components of the municipal regional food provisioning system in Cologne. Unique in this Delphi study is the bringing together of municipal administration, regional urban farmers and food activists. The research is still on-going, but preliminary results show that more communication among all relevant actors, especially horizontally among different city departments, in the urban food system is needed.
The Life Cycle Assessment (LCA) approach is the most important tool in the evaluation of environmental (sustainability) impacts of products and processes. We used the method to conduct an impact analysis with regard to raw material inputs (pulp) for the German paper production industry. In our analysis, we compare the environmental effects of primary sulphate pulp, scrap paper pulp and grass-based pulp and estimate their impacts in the impact categories "greenhouse gas emissions", "eutrophication" as well as "energy and water consumption". Furthermore, we discuss the opportunities of the methodical approach and some general problems and limits of the application of a LCA. In conclusion, we found environmental advantages for the use of grass as an alternative resource in the German paper production industry, especially in the fields of transport and water consumption.
Innovations in the mobility industry such as automated and connected cars could significantly reduce congestion and emissions by allowing the traffic to flow more freely and reducing the number of vehicles according to some researchers. However, the effectiveness of these sustainable product and service innovations is often limited by unexpected changes in consumption: some researchers thus hypothesize that the higher comfort and improved quality of time in driverless cars could lead to an increase in demand for driving with autonomous vehicles. So far, there is a lack of empirical evidence supporting either one or other of these hypotheses. To analyze the influence of autonomous driving on mobility behavior and to uncover user preferences, which serve as indicators for future travel mode choices, we conducted an online survey with a paired comparison of current and future travel modes with 302 participants in Germany. The results do not confirm the hypothesis that ownership will become an outdated model in the future. Instead they suggest that private cars, whether conventional or fully automated, will remain the preferred travel mode. At the same time, carsharing will benefit from full automation more than private cars. However, the findings indicate that the growth of carsharing will mainly be at the expense of public transport, showing that more emphasis should be placed in making public transport more attractive if sustainable mobility is to be developed.