The influence of simulated microgravity on purinergic signaling is different between individual culture and endothelial and smooth muscle cell coculture
- Exposure to microgravity conditions causes cardiovascular deconditioning in astronauts during spaceflight. Until now, no specific drugs are available for countermeasure, since the underlying mechanism is largely unknown. Endothelial cells (ECs) and smooth muscle cells (SMCs) play key roles in various vascular functions, many of which are regulated by purinergic 2 (P2) receptors. However, their function in ECs and SMCs under microgravity conditions is still unclear. In this study, primary ECs and SMCs were isolated from bovine aorta and verified with specific markers. We show for the first time that the P2 receptor expression pattern is altered in ECs and SMCs after 24 h exposure to simulated microgravity using a clinostat. However, conditioned medium compensates this change in specific P2 receptors, for example, P2X7. Notably, P2 receptors such as P2X7 might be the important players during the paracrine interaction. Additionally, ECs and SMCs secreted different cytokines under simulated microgravity, leading into a pathogenic proliferation and migration. In conclusion, our data indicate P2 receptors might be important players responding to gravity changes in ECs and SMCs. Since some artificial P2 receptor ligands are applied as drugs, it is reasonable to assume that they might be promising candidates against cardiovascular deconditioning in the future.
Document Type: | Article |
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Language: | English |
Author: | Yu Zhang, Patrick Lau, Andreas Pansky, Matthias Kassack, Ruth Hemmersbach, Edda Tobiasch |
Parent Title (English): | BioMed Research International |
Volume: | 2014 |
Article Number: | 413708 |
Number of pages: | 11 |
ISSN: | 2314-6133 |
ISSN: | 2314-6141 |
URN: | urn:nbn:de:hbz:1044-opus-13454 |
DOI: | https://doi.org/10.1155/2014/413708 |
PMID: | https://pubmed.ncbi.nlm.nih.gov/25243140 |
Publisher: | Hindawi |
Place of publication: | London |
Publishing Institution: | Hochschule Bonn-Rhein-Sieg |
Date of first publication: | 2014/08/28 |
Copyright: | © 2014 Yu Zhang et al. This is an open access article distributed under the Creative Commons Attribution License. |
Funding: | This work was supported by the Bundesministerium fur Bildung und Forschung- (BMBF-) FHprofUnt, [FKZ: 03FH012PB2 to E.T], NRW FH-Extra, [FKZ: z1112fh012 to E.T], DAAD PPP Vigoni, [FKZ: 314-vigoni-dr and FKZ: 54669218 to E.T], and BMBF-AIF, [FKZ: 1720X06 to E.T]; the fellowship of YZ was funded by China Scholarship Council [no. 20100602024] and the Helmholtz Space Life Sciences Research School (SpaceLife). SpaceLife is funded in equal parts by the Helmholtz Association and the German Aerospace Center (DLR). |
Departments, institutes and facilities: | Fachbereich Angewandte Naturwissenschaften |
Projects: | MeMoAthero - FHprofUnt2012: Mechanismus und Modell der Atherosklerose: Entwicklung eines Medikamententestsystems für einen neuartigen Behandlungsansatz (DE/BMBF/03FH012PB2,13FH012PB2) |
Untersuchung des Einflusses ausdifferenzierender Adipozyten bei der Pathogenese des Diabetes mellitus Typ 2 (AdiPaD) (DE/BMBF/1720X06;1320X06) | |
Dewey Decimal Classification (DDC): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Entry in this database: | 2015/04/02 |
Licence (German): | Creative Commons - CC BY - Namensnennung 3.0 |